The Poultry Health System
Ongoing Avian Influenza Survelliance
- PCR Testing Results Obtained within 2 Hours, Enabling a Rapid Response
- Determine the Epidemiology of the Disease
- Reimbursment by the Federal Government for Expenses Should Another Outbreak Occur
Chickens are submitted to Lasher Laboratory for routine exams (monitoring) or disease problem
- Ante mortem exam conducted – clinical signs
- Information collected – morbidity (disease) and mortality (death) rates
- Collect whole blood, serum or plasma for testing
- Post mortem exam (necropsy) conducted – record gross lesions and collect tissues for virus isolation, histopathology, or bacteriology using aseptic techniques
Samples from Lasher Laboratory are submitted to the Allen Laboratory and Worrilow Hall for processing In Vivo and In Vitro
- Tracheal swabs/ tissue samples for virus isolation in Specific Pathogen Free (SPF) embryonated eggs
- Serology – Hemmagglutination (HA), Hemmagglutination-Inhibition (HI), Agar Gel Precipitin test (AGP) and ELISA (Enzyme-Linked Immune Serum Assay) testing which normally measures antibodies for specific disease agents
- Formalin fixed tissues are processed and examined for microscopic lesions
- Egg Inoculations
- Chorioallantonic Sac (CAS)
- Yolk Sac (YS)
- Chorioallantonic Membrane
||Formalin Fixed Samples are Processed in Histology Laboratory In Worrilow Hall
|Slides are examined by a Poultry Pathologist, and Reports are Submitted to Clients by Phone, Hard Copy or E-mail.
- Infectious agents are isolated, identified and used as antigens in experimental vaccines.
- Live, inactivated or genetically engineered vaccines are tested for their ability to stimulate protective immune response (induction of acquired immunity).
Certain organisms which can cause disease (biohazard) require bio- containment.
The biosafety level (BSL) of Biocontainment varies with organisms used.
- BSL-2 for field accessions samples from Lasher Lab
- BSL-3 for Avian Influenza, recombinant agents and exotic agents